Comparison of an in vitro skin model to normal human skin for dermatological research

Author(s):  
Nancy A. Monteiro-Riviere ◽  
Alfred O. Inman ◽  
Thomas H. Snider ◽  
Jim A. Blank ◽  
David W. Hobson
1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.


1998 ◽  
Vol 37 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Vincenzo Ruocco ◽  
Sarah Brenner ◽  
Eleonora Ruocco ◽  
Francesca De Angelis ◽  
Maria Luisa Lombardi

Phytomedicine ◽  
2012 ◽  
Vol 19 (13) ◽  
pp. 1223-1227 ◽  
Author(s):  
Jeong-Hyun Lee ◽  
Hye-Lee Kim ◽  
Mi Hee Lee ◽  
Kyung Eun You ◽  
Byeong-Ju Kwon ◽  
...  

1994 ◽  
Vol 303 (3) ◽  
pp. 863-867 ◽  
Author(s):  
D Chatellard-Gruaz ◽  
J H Saurat ◽  
G Siegenthaler

Cyclophilin A, the major intracellular binding protein for the immunosuppressive drug cyclosporin A (CsA), was studied in human keratinocytes during differentiation both in vivo and in vitro. Analysis of cyclophilin by gel-filtration radiobinding-assay with tritiated CsA showed one specific radioactive peak at 17 kDa. By this technique, the levels of cyclophilin (mean 55.23 +/- 8.43 pmol/mg protein) did not significantly differ during keratinocyte differentiation. When the protein extracts from calcium-induced differentiating keratinocytes and normal human skin were analysed by PAGE radiobinding-assay, two specific radioactive CsA-binding peaks were detected. The major peak (RF 0.13) was expressed in all samples (mean 47.32 +/- 17.53 pmol/mg protein) whereas the minor peak (RF 0.23) was dramatically decreased about 6-fold in abnormally differentiated skin (psoriasis) as well as in non-differentiated keratinocytes. At least six [3H]CsA-binding isoforms with pI values ranging from 5.58 to 7.75 were detected by isoelectrofocusing autoradio-blotting-assay in normal human skin; three of them immunoreacted with antibodies to cyclophilin. These results demonstrated the presence of several cyclophilin isoforms in human epidermal cells and an expression which correlated with the differentiation of human keratinocytes both in vivo and in vitro.


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